OS-Seq

Oligonucleotide-Selective Sequencing

OS-Seq was developed to improve targeted resequencing by capturing and sequencing gene targets directly on the flow cell (Myllykangas et al., 2011). In this method, target sequences with adapters are used to modify the flow cell primers. Targets in the template are captured onto the flow cell with the modified primers. Further extension, denaturation, and hybridization provide sequence reads for the target genes. Deep sequencing provides accurate representation of reads.

Advantages:

• Can resequence multiple targets at a time
• No gel excision or narrow size-purification required
• Rapid, single-day protocol
• Samples can be multiplexed
• Reduced PCR bias due to removal of amplification steps
• Avoids loss of material

Disadvantages:

• Primers may interact with similar target sequences, leading to sequence ambiguity

Reagents:

Illumina Library prep and Array Kit Selector



Reviews:

None available yet

References:

Akinrinade O., Ollila L., Vattulainen S., et al. Genetics and genotype-phenotype correlations in Finnish patients with dilated cardiomyopathy. Eur Heart J. 2015;36:2327-2337

Vattulainen S., Aho J., Salmenpera P., et al. Accurate genetic diagnosis of Finnish pulmonary arterial hypertension patients using oligonucleotide-selective sequencing. Mol Genet Genomic Med. 2015;3:354-362

Hopmans E. S., Natsoulis G., Bell J. M., Grimes S. M., Sieh W. and Ji H. P. A programmable method for massively parallel targeted sequencing. Nucleic Acids Res. 2014;42:e88

Edit: OS-Seq

You must be logged in to edit this entry.

History: OS-Seq

Revision by sbrumpton on 2017-06-21 07:50:21 - Show/Hide