Multiple Annealing and Looping_Based Amplification Cycles

MALBAC is intended to address some of the shortcomings of MDA (Zong et al., 2012). In this method, MALBAC primers randomly anneal to a DNA template. A polymerase with displacement activity at elevated temperatures amplifies the template, generating –semiamplicons.” As the amplification and annealing process is repeated, the semiamplicons are amplified into full amplicons that have a 3ê end complementary to the 5ê end. As a result, full-amplicon ends hybridize to form a looped structure, inhibiting further amplification of the looped amplicon, while only the semiamplicons and gDNA undergo amplification. Deep sequencing of the full-amplicon sequences allows for accurate representation of reads, while sequencing depth provides improved alignment for consensus sequences . This method can also be applied to cDNA for transcriptome analysis (Briese et al., 2016).


  • Can sequence large templates
  • Can perform single-cell sequencing or sequencing for samples with limited amounts of starting material
  • Full-amplicon looping inhibits overrepresentation of templates, reducing PCR bias
  • Can amplify GC-rich regions
  • Provides uniform genome coverage
  • Lower allele dropout rate compared to MDA



Illumina Library prep and Array Kit Selector


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