5-Formylcytosine Chemical Modification_Assisted Bisulfite Sequencing

fCAB-Seq is a method for EtONH2-modified bisulfite sequencing for base-resolution detection of 5fC in gDNA (Song et al., 2013). It is complementary to fC-Seal, which was developed as a highly selective chemical labeling approach for the affinity purification and genome-wide profiling of 5fC (Neri et al., 2016)


  • Single-base resolution



Illumina Library prep and Array Kit Selector


Balasubramanian S. Chemical biology on the genome. Bioorg Med Chem. 2014;22:4356-4370

Booth M. J., Marsico G., Bachman M., Beraldi D. and Balasubramanian S. Quantitative sequencing of 5-formylcytosine in DNA at single-base resolution. Nat Chem. 2014;6:435-440

Rivera C. M. and Ren B. Mapping human epigenomes. Cell. 2013;155:39-55


Wang L., Zhang J., Duan J., et al. Programming and inheritance of parental DNA methylomes in mammals. Cell. 2014;157:979-991

Van Haute L., Dietmann S., Kremer L., Hussain S., Pearce S. F., et al. Deficient methylation and formylation of mt-tRNA(Met) wobble cytosine in a patient carrying mutations in NSUN3. Nat Commun. 2016;7:12039

Xia B., Han D., Lu X., Sun Z., Zhou A., et al. Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale. Nat Methods. 2015;12:1047-1050

Becker D., Lutsik P., Ebert P., Bock C., Lengauer T. and Walter J. BiQ Analyzer HiMod: an interactive software tool for high-throughput locus-specific analysis of 5-methylcytosine and its oxidized derivatives. Nucleic Acids Res. 2014;42:W501-507