Circular Chromatin Conformation Capture

4C (Zhao et al., 2006), (Simonis et al., 2006), also called 4C-seq, is a method similar to 3C and is sometimes called circular 3C. It allows the unbiased detection of all genomic regions that interact with a particular region of interest (Sajan et al., 2012). In this method, DNA-protein complexes are crosslinked using formaldehyde. The sample is fragmented, and the DNA is ligated and digested. The resulting DNA fragments self-circularize, followed by reverse PCR and sequencing. Deep sequencing provides base-pair resolution of the ligated fragments.


  • Preferred strategy to assess the DNA contact profile of individual genomic sites
  • Highly reproducible data


  • Will miss local interactions (< 50 kb) from the region of interest
  • Large circles do not amplify efficiently

Illumina Library prep and Array Kit Selector

None available yet
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