Map DNA Single-Strand Breaks
SSB-Seq maps in vivo DNA single-strand breaks (SSBs) on a genome-wide scale (Baranello et al., 2014).In this method, gDNA is isolated and subjected to nick-translation by DNA polymerase I in the presence of deoxynucleotide triphosphates (dNTPs), including digoxigenin-labeled dUTP, and a small amount of dideoxynucleotides. The DNA is sheared, and the labeled fragments are immunoprecipitated with antidigoxigenin antibodies and purified to prepare a sequencing library.
- Simple protocol
- Locations of breaks cannot be determined accurately
- Not yet adopted widely by the scientific community
Illumina Library prep and Array Kit Selector
None available yet
Baranello L., Kouzine F., Wojtowicz D., et al. DNA break mapping reveals topoisomerase II activity genome-wide. Int J Mol Sci. 2014;15:13111-13122