LAM-HTGTS
Linear Amplification-Mediated High-Throughput Genome-Wide Sequencing
LAM-HTGTS is a method for the genome-wide detection of –prey” DSBs via their translocation to a fixed –bait” DSB in cultured mammalian cells (Hu et al., 2016). Bait-prey junctions are cloned directly from isolated gDNA using LAM-PCR and ligated unidirectionally to bridge adapters. Subsequent PCR steps amplify the single-stranded DNA junction library in preparation for sequencing.
Advantages:
- Sensitive detection of DSBs within chromosomes
Disadvantages:
- Not applicable to limited amounts of material
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
None available yet
References:
Hu J., Meyers R. M., Dong J., Panchakshari R. A., Alt F. W., et al. Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing. Nat Protoc. 2016;11:853-871