Linear Amplification-Mediated High-Throughput Genome-Wide Sequencing
LAM-HTGTS is a method for the genome-wide detection of –prey” DSBs via their translocation to a fixed –bait” DSB in cultured mammalian cells (Hu et al., 2016). Bait-prey junctions are cloned directly from isolated gDNA using LAM-PCR and ligated unidirectionally to bridge adapters. Subsequent PCR steps amplify the single-stranded DNA junction library in preparation for sequencing.
- Sensitive detection of DSBs within chromosomes
- Not applicable to limited amounts of material
Illumina Library prep and Array Kit Selector
None available yet
Hu J., Meyers R. M., Dong J., Panchakshari R. A., Alt F. W., et al. Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing. Nat Protoc. 2016;11:853-871