CXXC Affinity Purification Sequencing

CAP-seq maps methylated CpGs in gDNA (Illingworth et al., 2010). In this method, DNA is incubated with CXXC bound to nickel-charged sepharose beads. Next, DNA is eluted from the complex and sequenced. Deep sequencing provides insights into methylated CpG sites in gDNA.


  • Targets CpG islands


  • None known


Illumina Library prep and Array Kit Selector


None available yet


Vilborg A., Passarelli M. C., Yario T. A., Tycowski K. T. and Steitz J. A. Widespread Inducible Transcription Downstream of Human Genes. Mol Cell. 2015;59:449-461

Smallwood S. A., Lee H. J., Angermueller C., et al. Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity. Nat Methods. 2014;11:817-820

Suzuki M. M., Yoshinari A., Obara M., et al. Identical sets of methylated and nonmethylated genes in Ciona intestinalis sperm and muscle cells. Epigenetics Chromatin. 2013;6:38