Hybridization RAD for Degraded DNA
hyRAD (Suchan et al., 2016) was developed for use on degraded DNA samples, such as those from museum collections. Museum and preserved samples offer a rich source of valuable specimens, but their degraded DNA is unable to sustain the double-digestion and sample fractionation required by ddRAD (Peterson et al., 2012). To address this limitation, hyRAD uses biotinylated probes and streptavidin-covered beads to capture and enrich the fragments of interest.
The first step in the process is to generate a ddRAD library from high-quality DNA, usually from an extant specimen. The fragments are size-selected and biotinylated. They can now be used as probes for hybridization capture of shotgun or ddRad libraries.
- Can be used on degraded DNA
- Can be used on unsequenced genomes
- Coverage not as complete as with high-quality samples
Illumina Library prep and Array Kit Selector
Andrews K. R., Good J. M., Miller M. R., Luikart G. and Hohenlohe P. A. Harnessing the power of RADseq for ecological and evolutionary genomics. Nat Rev Genet. 2016;
Holmes M. W., Hammond T. T., Wogan G. O., et al. Natural history collections as windows on evolutionary processes. Mol Ecol. 2016;25:864-881
Suchan T., Pitteloud C., Gerasimova N. S., et al. Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens. PLoS One. 2016;11:e0151651