Microwell displacement amplification system/Immunomagnetic Separation for Targeted Bacterial Enrichment for MDA/Digital Droplet MDA

Several variations on the original MDA methodã (Dean et al., 2001) such as MIDAS (Gole et al., 2013), ddMDA (Rhee et al., 2016), SNES (Leung et al., 2015), and IMS-MDA (Seth-Smith et al., 2013) ãhave been developed to improve the amplification bias and throughput (Seth-Smith et al., 2013).

Microwell and droplet displacement amplification systems are protocol variations of MDA in which single cells are randomly distributed into hundreds to thousands of nanoliter wells or droplets, and their genetic material is simultaneously amplified (Gole et al., 2013). Sequencing individual cells simplifies the genomic diversity and reduces inherent amplification biases among cells. It results in improved representation and sensitivity in heterogeneous cell populations. In an alternative approach to simplify genomic diversity, IMS-MDA uses immunomagnetic separation for targeted bacterial enrichment (Seth-Smith et al., 2013).


  • Templates used for this method can be circular DNA (eg, plasmids, bacterial DNA)
  • Can sequence large templates
  • Can perform single-cell sequencing or sequencing for samples with limited amounts of starting material


  • PCR biases can underrepresent GC-rich templates


Illumina Library prep and Array Kit Selector


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