Genome and Transcriptome Sequencing

G&T-seq can separate and sequence gDNA and full-length mRNA from single cells (Macaulay et al., 2015). In this method, single cells are isolated and lysed. RNA is captured using biotinylated oligo(dT) capture primers and separated from DNA using streptavidin-coated magnetic beads. Smart-seq2 is used to amplify captured RNA on the bead, while MDA is used to amplify DNA. After sequencing, integrating the DNA and RNA sequences provides insights into the gene expression profiles of single cells.


  • Compatible with any WGA method
  • No 3ê-end bias in sequence reads because full-length transcripts are captured
  • Because DNA and RNA are physically separated and amplified independently, there is no need to mask coding sequences during analysis


  • Physical separation of DNA and RNA can increase risk of sample loss or contamination
  • Increased handling time


Illumina Library prep and Array Kit Selector


Zhang X., Marjani S. L., Hu Z., Weissman S. M., Pan X., et al. Single-Cell Sequencing for Precise Cancer Research: Progress and Prospects. Cancer Res. 2016;76:1305-1312

Saadatpour A., Lai S., Guo G. and Yuan G. C. Single-Cell Analysis in Cancer Genomics. Trends Genet. 2015;31:576-586


Macaulay I. C., Haerty W., Kumar P., et al. G&T-seq: parallel sequencing of single-cell genomes and transcriptomes. Nat Methods. 2015;12:519-522