Tet-Assisted Bisulfite Sequencing

TAB-Seq is a novel method that uses bisulfite conversion and Tet proteins to study 5hmC (Yu et al., 2012). In this method, 5hmC is first protected selectively with a glucose moiety, followed by subsequent oxidation of 5mC to 5caC by Tet proteins. Next, the oxidized gDNA is treated with bisulfite; 5hmC remains unchanged and is read as a cytosine, while 5mC and unmethylated cytosines are deaminated to uracil and read as thymidines upon sequencing. Deep sequencing of TAB-treated DNA compared with untreated DNA provides accurate representation of 5hmC localization in the genome.


  • Covers CpG and non-CpG hydroxymethylation throughout the genome at single-base resolution
  • Covers 5hmC in dense and less dense repeat regions
  • Clearly differentiates between 5hmC and 5mC, specifically identifying 5hmC


  • Harsh oxidation can lead to substantial loss of DNA


Illumina Library prep and Array Kit Selector


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