RRMAB-seq
Reduced-Representation M.SssI Methylase-Assisted Bisulfite Sequencing
RRMAB-seq is based on the pretreatment of gDNA with the enzyme MspI, which recognizes and cuts the CCGG consensus to enrich CpG-rich regions, like gene promoters (Neri et al., 2015). This method is an adapted version of MAB-seq (Wu et al., 2014)
Advantages:
- Increased coverage of CpG-rich regions reduces sequencing costs and increases read depth
- Reduced number of chemical or enzymatic DNA treatments required before bisulfite treatment
Disadvantages:
- Unable to distinguish 5fC/5caC from unmodified C within a non-CpG context (Neri et al., 2015)
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
None available yet
References:
Neri F., Incarnato D., Krepelova A., Parlato C. and Oliviero S. Methylation-assisted bisulfite sequencing to simultaneously map 5fC and 5caC on a genome-wide scale for DNA demethylation analysis. Nat Protoc. 2016;11:1191-1205