Reduced-Representation Bisulfite Sequencing

RRBS uses one or multiple restriction enzymes on the genomic DNA to produce sequence-specific fragmentation (Meissner et al., 2005). The fragmented genomic DNA is treated with bisulfite and sequenced. This is the method of choice to study specific regions of interest. It is particularly effective where methylation levels are high, such as in promoters and repeat regions.


  • Provides genome-wide coverage of CpGs in islands at single-base resolution
  • Covers CpG methylation in dense regions


  • Restriction enzymes cut at specific sites, providing biased sequence selection
  • Method measures 10-15% of all CpGs in the genome
  • Cannot distinguish between 5mC and 5hmC
  • Does not cover non-CpG areas, genome-wide CpGs, and CpGs in areas without the enzyme restriction site


Illumina Library prep and Array Kit Selector


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