EpiRADseq

Double-Digest Restriction Site_Associated DNA Marker Generation with a Methylation-Sensitive Restriction Enzyme

EpiRADseq is similar to the widely used double-digest RAD-seq method (ddRADseq), except that it uses an MSRE Schield et al., 2016). DNA samples are digested with PstI and HpaII followed by purification. Double-stranded sequencing adapters with unique barcodes are ligated to each sample. Size-selected libraries are then amplified with indexed primers.

Advantages:

  • Does not need a reference genome

Disadvantages:

  • Measures a subset of methylation sites


Reagents:

Illumina Library prep and Array Kit Selector



Reviews:

None available yet

References:

Schield D. R., Walsh M. R., Card D. C., et al. EpiRADseq: scalable analysis of genomewide patterns of methylation using next-generation sequencing. Methods in Ecology and Evolution. 2016;7:60-69