BSPP

Bisulfite Sequencing With Padlock Probes

BSPP is a targeted method that isolates selected locations for methylation profiling (Deng et al., 2009) (Ball et al., 2009). Padlock probes are ~100 nt DNA fragments designed to hybridize to genomic DNA targets in a horseshoe manner. After the gap between the 2 hybridized, locus-specific arms of a padlock probe is filled and ligated, the circular strand of DNA can be amplified and sequenced.165

Advantages:

  • Requires only 100 ng of DNA for amplification-free WGBS of mammalian genomes

Disadvantages:


Reagents:

Illumina Library prep and Array Kit Selector



Reviews:

Shull A. Y., Noonepalle S. K., Lee E. J., Choi J. H. and Shi H. Sequencing the cancer methylome. Methods Mol Biol. 2015;1238:627-651



References:

Diep D., Plongthongkum N., Gore A., Fung H. L., Shoemaker R. and Zhang K. Library-free methylation sequencing with bisulfite padlock probes. Nat Methods. 2012;9:270-272