Bisulfite Sequencing With Padlock Probes

BSPP is a targeted method that isolates selected locations for methylation profiling (Deng et al., 2009) (Ball et al., 2009). Padlock probes are ~100 nt DNA fragments designed to hybridize to genomic DNA targets in a horseshoe manner. After the gap between the 2 hybridized, locus-specific arms of a padlock probe is filled and ligated, the circular strand of DNA can be amplified and sequenced.165


  • Requires only 100 ng of DNA for amplification-free WGBS of mammalian genomes



Illumina Library prep and Array Kit Selector


Shull A. Y., Noonepalle S. K., Lee E. J., Choi J. H. and Shi H. Sequencing the cancer methylome. Methods Mol Biol. 2015;1238:627-651


Diep D., Plongthongkum N., Gore A., Fung H. L., Shoemaker R. and Zhang K. Library-free methylation sequencing with bisulfite padlock probes. Nat Methods. 2012;9:270-272