Bisulfite Amplicon Sequencing

BSAS is a targeted BS-Seq method that uses PCR enrichment of targeted regions and transposome-mediated library construction for rapid generation of sequencing libraries, from low (1 ng) sample input (Masser et al., 2013).Genomic DNA is bisulfite-converted and subjected to PCR, using primers specific for bisulfite-converted DNA. The amplicons are subjected to Nextera XT library preparation, including dual indexing. The final libraries consist of a random insert of bisulfite-converted amplified DNA, capture probes, and specific index sequences. These libraries are multiplexed and sequenced.


  • Can be applied to any genomic region from any DNA source, including tissue and cell culture.
  • Rapid and highly quantitative


  • Does not cover the whole genome
  • Genome and target must be known


Illumina Library prep and Array Kit Selector


None available yet


Sun L., Wang J., Yin X., et al. Identification of a 5-Methylcytosine Site that may Regulate C/EBPbeta Binding and Determine Tissue-Specific Expression of the BPI Gene in Piglets. Sci Rep. 2016;6:28506

Ou X., Thakali K. M., Shankar K., Andres A. and Badger T. M. Maternal adiposity negatively influences infant brain white matter development. Obesity (Silver Spring). 2015;23:1047-1054