AbaSI Coupled with Sequencing

Aba-seq is a method for high-resolution mapping of the 5hmC methylome that provides sensitive detection of 5hmC at low-occupancy regions (Sun et al., 2013). The method relies on the unique property of the restriction enzyme AbaSI to recognize glucosylated 5hmC with high specificity, compared to 5mC and C. A single-cell variation, scAba-seq, is also available (Mooijman et al., 2016). A related method uses PvuRts1I to overcome the sequence bias in AbaSI (Sun et al., 2015)


  • Covers CpG and non-CpG methylation throughout the genome at single-base resolution
  • Covers 5mC in dense and less dense repeat regions
  • Clearly differentiates between 5mC and 5hmC



Illumina Library prep and Array Kit Selector


Plongthongkum N., Diep D. H. and Zhang K. Advances in the profiling of DNA modifications: cytosine methylation and beyond. Nat Rev Genet. 2014;15:647-661


Sun Z., Terragni J., Borgaro J. G., et al. High-resolution enzymatic mapping of genomic 5-hydroxymethylcytosine in mouse embryonic stem cells. Cell Rep. 2013;3:567-576