Mapping in Vivo Nascent Chromatin with EdU
MINCE-seq was developed to characterize the genome-wide location of nucleosomes and other chromatin proteins behind replication forks at high temporal and spatial resolution (Ramachandran et al., 2016).
In this method, newly replicated DNA is labeled with the nucleotide analog EdU, which is coupled with biotin using click chemistry. Coupling with biotin ensures highly specific purification of newly replicated DNA from asynchronous cells, even if it is only a fraction of a percentage of total DNA. Subsequent MNase treatment recovers DNA fragments bound both by nucleosomes and by nonhistone DNA-binding proteins, which enables the mapping of newly replicated chromatin at near base-pair resolution.
- Maps newly replicated chromatin at near base-pair resolution
- Robustness unknown
Illumina Library prep and Array Kit Selector
None available yet
Ramachandran S. and Henikoff S. Transcriptional Regulators Compete with Nucleosomes Post-replication. Cell. 2016;165:580-592