Assay for Transposase-Accessible Chromatin Sequencing / ATAC-seq Optimized for Blood Cells

ATAC-Seq uses the Tn5 transposome to detect nucleosome-free regions of the genome (Buenrostro et al., 2013). The method is commonly used, and optimized protocols are available for tissues, such as blood (Fast-ATAC) (Corces et al., 2016), neurons (Milani et al., 2016), biobank specimens (Scharer et al., 2016), and single cells (scATAC-seq (Buenrostro et al., 2015) and single-cell ATAC-seq (Cusanovich et al., 2015) ).

In this method, gDNA is incubated with Tn5 transposomes, which fragments it and adds adapters simultaneously, in open chromatin regions. Deep sequencing of the purified regions provides base-pair resolution of nucleosome-free regions in the genome.


  • Two-step protocol with no adapter ligation steps, gel purification, or crosslink reversal
  • High signal-to-noise ratio compared to FAIRE-Seq


  • During mechanical sample processing, bound chromatin regions might open and be tagged by the transposome
  • Only half of the molecules contain the adapters in the orientation required for PCR amplification
  • Distance between adapter sites may not be optimal for PCR amplification (Sos et al., 2016)


Illumina Library prep and Array Kit Selector


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