MeRIP-Seq

m6A-Specific Methylated RNA Immunoprecipitation Sequencing

MeRIP-Seq maps m6A-methylated RNA. In this method, m6A-specific antibodies are used to immunoprecipitate RNA. RNA is reverse-transcribed to cDNA and sequenced. Deep sequencing provides high-resolution reads of m6A-methylated RNA (Meyer et al., 2012)

Similar methods: miCLIP, m6A-LAIC-Seq

Advantages:

  • Maps m6A-methylated RNA

Disadvantages:

  • Antibodies not specific to the target will precipitate nonspecific RNA modifications.


Reagents:

Illumina Library prep and Array Kit Selector



Reviews:

Bian Q. and Cahan P. Computational Tools for Stem Cell Biology. Trends Biotechnol. 2016;



References:

Gokhale N. S., McIntyre A. B., McFadden M. J., et al. N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection. Cell Host Microbe. 2016;