MeRIP-Seq
m6A-Specific Methylated RNA Immunoprecipitation Sequencing
MeRIP-Seq maps m6A-methylated RNA. In this method, m6A-specific antibodies are used to immunoprecipitate RNA. RNA is reverse-transcribed to cDNA and sequenced. Deep sequencing provides high-resolution reads of m6A-methylated RNA (Meyer et al., 2012)
Similar methods: miCLIP, m6A-LAIC-Seq
Advantages:
- Maps m6A-methylated RNA
Disadvantages:
- Antibodies not specific to the target will precipitate nonspecific RNA modifications.
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
Bian Q. and Cahan P. Computational Tools for Stem Cell Biology. Trends Biotechnol. 2016;
References:
Gokhale N. S., McIntyre A. B., McFadden M. J., et al. N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection. Cell Host Microbe. 2016;