Transcriptome in Vivo Analysis

TIVA is a protocol that captures mRNA from live cells (Lovatt et al., 2014). In this method, a photoactivatable TIVA tag is loaded into cells. Selective photoactivation exposes the mRNA-capturing portion of the tag, allowing it to hybridize to the poly(A) tails of mRNA. The biotin-bound mRNA is captured using streptavidin-coated magnetic beads and transcribed into cDNA. Sequencing the cDNA provides transcriptome analysis of RNA from single cells in complex tissues.


  • in vivo transcriptome analysis using photoactivation in neuronal cells
  • Noninvasive method for capturing mRNAs in their natural microenvironment


  • Limited to a small number of cells


Illumina Library prep and Array Kit Selector


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