Excision Circle Sequencing
EC-seq allows the capture and analysis of immune locus rearrangements from whole thymic and splenic tissues(Parkinson et al., 2015) .Double-stranded cleavage at recombination signal sequence (RSS) sites, mediated by recombination-activating gene recombinase (RAG), precedes genomic deletion and end-processing by the nonhomologous end-joining (NHEJ) complex. Coding ends are covalently hair-pinned and reopened prior to ligation. The excision circle junction is then ligated from unprocessed DNA ends with little modification and used to prepare a sequencing library.
- Provides capture and analysis of immune locus rearrangements
- Not yet adopted widely by the scientific community
Illumina Library prep and Array Kit Selector
None available yet
Parkinson N. J., Roddis M., Ferneyhough B., et al. Violation of the 12/23 rule of genomic V(D)J recombination is common in lymphocytes. Genome Res. 2015;25:226-234