EC-seq
Excision Circle Sequencing
EC-seq allows the capture and analysis of immune locus rearrangements from whole thymic and splenic tissues(Parkinson et al., 2015) .Double-stranded cleavage at recombination signal sequence (RSS) sites, mediated by recombination-activating gene recombinase (RAG), precedes genomic deletion and end-processing by the nonhomologous end-joining (NHEJ) complex. Coding ends are covalently hair-pinned and reopened prior to ligation. The excision circle junction is then ligated from unprocessed DNA ends with little modification and used to prepare a sequencing library.
Advantages:
- Provides capture and analysis of immune locus rearrangements
Disadvantages:
- Not yet adopted widely by the scientific community
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
None available yet
References:
Parkinson N. J., Roddis M., Ferneyhough B., et al. Violation of the 12/23 rule of genomic V(D)J recombination is common in lymphocytes. Genome Res. 2015;25:226-234