PD-Seq
Candidate Cellular Protein Target Identification
PD-Seq is a protocol to identify cellular targets for proteins through protein-protein interactions (Arango et al., 2013). In this method, researchers identified cellular targets for apigenin. They coupled apigenin to amino polyethyleneglycol-polyacrylamide copolymer (PEGA) beads after activation with 4-nitrophenyl bromoacetate. The apigenin-loaded beads were used for screening phage display cDNA library generated using mRNA from human breast cancer tumor cells. After 3 rounds, the fractions were used to make libraries and sequenced on the Illumina platform.
Advantages:
- Can detect a large number of cellular targets
Disadvantages:
- Not yet adopted widely by the scientific community
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
None available yet
References:
Arango D., Morohashi K., Yilmaz A., et al. Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets. Proc Natl Acad Sci U S A. 2013;110:E2153-2162