Candidate Cellular Protein Target Identification
PD-Seq is a protocol to identify cellular targets for proteins through protein-protein interactions (Arango et al., 2013). In this method, researchers identified cellular targets for apigenin. They coupled apigenin to amino polyethyleneglycol-polyacrylamide copolymer (PEGA) beads after activation with 4-nitrophenyl bromoacetate. The apigenin-loaded beads were used for screening phage display cDNA library generated using mRNA from human breast cancer tumor cells. After 3 rounds, the fractions were used to make libraries and sequenced on the Illumina platform.
- Can detect a large number of cellular targets
- Not yet adopted widely by the scientific community
Illumina Library prep and Array Kit Selector
None available yet
Arango D., Morohashi K., Yilmaz A., et al. Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets. Proc Natl Acad Sci U S A. 2013;110:E2153-2162