Multiple Displacement Amplification

MDA is a method commonly used for sequencing microbial genomes due to its ability to amplify templates larger than 0.5 Mbp, but it can also be used to study genomes of other sizes (Dean et al., 2001). In this method, 3ê-blocked random hexamer primers are hybridized to the template, followed by strand-displacement DNA synthesis with Phi 29 polymerase. The method allows for efficient and rapid DNA amplification. Deep sequencing of the amplified DNA provides accurate representation of reads, while sequencing depth provides better alignment and consensus for sequences. Several variations on the original MDA methodãsuch as MIDAS (Gole et al., 2013), ddMDA (Rhee et al., 2016), SNES (Leung et al., 2015), and IMS-MDA (Seth-Smith et al., 2013) ãhave been developed to improve the amplification bias and throughput (Seth-Smith et al., 2013).


  • Templates can be circular DNA (eg, plasmids, bacterial DNA)
  • Can sequence large templates
  • Can perform single-cell sequencing or sequencing for samples with limited amounts of starting material



Illumina Library prep and Array Kit Selector


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