M.SssI Methylase-Assisted Bisulfite Sequencing
MAB-seq allows simultaneous and quantitative mapping of both 5fC and 5caC at single-base resolution (Wu et al., 2014). This method is complementary to caMAB-seq, a method for direct 5caC mapping.In this method, gDNA is treated with the bacterial CpG methyltransferase M.SssI, which methylates CpG dinucleotides. Next, bisulfite conversion of methylase-treated DNA leads to deamination of only 5fC and 5caC; originally unmodified CpGs are protected as 5mCpG. After sequencing, 5fC and 5caC are read as thymidine, while 5mC is read as cytosine.
- Can provide a quantitative measurement of the abundance of 5fC/5caC within CpG dyads.
- Unable to distinguish 5fC/5caC from unmodified C within a non-CpG context
Illumina Library prep and Array Kit Selector
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