Double-Digest Restriction Site_Associated DNA Marker Generation with a Methylation-Sensitive Restriction Enzyme
EpiRADseq is similar to the widely used double-digest RAD-seq method (ddRADseq), except that it uses an MSRE Schield et al., 2016). DNA samples are digested with PstI and HpaII followed by purification. Double-stranded sequencing adapters with unique barcodes are ligated to each sample. Size-selected libraries are then amplified with indexed primers.
- Does not need a reference genome
- Measures a subset of methylation sites
Illumina Library prep and Array Kit Selector
None available yet
Schield D. R., Walsh M. R., Card D. C., et al. EpiRADseq: scalable analysis of genomewide patterns of methylation using next-generation sequencing. Methods in Ecology and Evolution. 2016;7:60-69