Chemical Modification_Assisted Bisulfite Sequencing

CAB-Seq can detect 5caC with single-base resolution in DNA (Lu et al., 2013). It is based on the observation that chemically modified 5caC can survive bisulfite treatment without deamination.


  • Single-base resolution


  • Relatively low protection rate of 5caC deamination (50_60%) (Wu et al., 2014)
  • Requires the sequencing of a nontreated DNA control, and the calling of the modified cytosines is based on a subtractive analysis; requires high sequencing depth to reduce the false-discovery rate


Illumina Library prep and Array Kit Selector


Plongthongkum N., Diep D. H. and Zhang K. Advances in the profiling of DNA modifications: cytosine methylation and beyond. Nat Rev Genet. 2014;15:647-661


Lu X., Song C. X., Szulwach K., et al. Chemical modification-assisted bisulfite sequencing (CAB-Seq) for 5-carboxylcytosine detection in DNA. J Am Chem Soc. 2013;135:9315-9317