BSPP
Bisulfite Sequencing With Padlock Probes
BSPP is a targeted method that isolates selected locations for methylation profiling (Deng et al., 2009) (Ball et al., 2009). Padlock probes are ~100 nt DNA fragments designed to hybridize to genomic DNA targets in a horseshoe manner. After the gap between the 2 hybridized, locus-specific arms of a padlock probe is filled and ligated, the circular strand of DNA can be amplified and sequenced.165
Advantages:
- Requires only 100 ng of DNA for amplification-free WGBS of mammalian genomes
Disadvantages:
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
Shull A. Y., Noonepalle S. K., Lee E. J., Choi J. H. and Shi H. Sequencing the cancer methylome. Methods Mol Biol. 2015;1238:627-651
References:
Diep D., Plongthongkum N., Gore A., Fung H. L., Shoemaker R. and Zhang K. Library-free methylation sequencing with bisulfite padlock probes. Nat Methods. 2012;9:270-272
Related
History: BSPP
Revision by on 2017-06-21 07:50:24 - Show/Hide
Bisulfite Sequencing With Padlock Probes
BSPP is a targeted method that isolates selected locations for methylation profiling (Deng et al., 2009) (Ball et al., 2009). Padlock probes are ~100 nt DNA fragments designed to hybridize to genomic DNA targets in a horseshoe manner. After the gap between the 2 hybridized, locus-specific arms of a padlock probe is filled and ligated, the circular strand of DNA can be amplified and sequenced.165
Advantages:- Requires only 100 ng of DNA for amplification-free WGBS of mammalian genomes
Disadvantages:Reagents:Illumina Library prep and Array Kit SelectorReviews:Shull A. Y., Noonepalle S. K., Lee E. J., Choi J. H. and Shi H. Sequencing the cancer methylome. Methods Mol Biol. 2015;1238:627-651References:Diep D., Plongthongkum N., Gore A., Fung H. L., Shoemaker R. and Zhang K. Library-free methylation sequencing with bisulfite padlock probes. Nat Methods. 2012;9:270-272Revision by sbrumpton on 2017-06-21 07:50:20 - Show/Hide
Bisulfite Sequencing With Padlock Probes
BSPP is a targeted method that isolates selected locations for methylation profiling (Deng et al., 2009) (Ball et al., 2009). Padlock probes are ~100 nt DNA fragments designed to hybridize to genomic DNA targets in a horseshoe manner. After the gap between the 2 hybridized, locus-specific arms of a padlock probe is filled and ligated, the circular strand of DNA can be amplified and sequenced.165
Advantages:- Requires only 100 ng of DNA for amplification-free WGBS of mammalian genomes
Disadvantages:Reagents:Illumina Library prep and Array Kit SelectorReviews:Shull A. Y., Noonepalle S. K., Lee E. J., Choi J. H. and Shi H. Sequencing the cancer methylome. Methods Mol Biol. 2015;1238:627-651References:Diep D., Plongthongkum N., Gore A., Fung H. L., Shoemaker R. and Zhang K. Library-free methylation sequencing with bisulfite padlock probes. Nat Methods. 2012;9:270-272