Bisulfite Amplicon Sequencing
BSAS is a targeted BS-Seq method that uses PCR enrichment of targeted regions and transposome-mediated library construction for rapid generation of sequencing libraries, from low (1 ng) sample input (Masser et al., 2013).Genomic DNA is bisulfite-converted and subjected to PCR, using primers specific for bisulfite-converted DNA. The amplicons are subjected to Nextera XT library preparation, including dual indexing. The final libraries consist of a random insert of bisulfite-converted amplified DNA, capture probes, and specific index sequences. These libraries are multiplexed and sequenced.
- Can be applied to any genomic region from any DNA source, including tissue and cell culture.
- Rapid and highly quantitative
- Does not cover the whole genome
- Genome and target must be known
Illumina Library prep and Array Kit Selector
None available yet
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