THS-seq
Transposome Hypersensitive Sites Sequencing
THS-seq is a method for highly sensitive characterization of chromatin accessibility. This variation on ATAC-seq uses linear amplification of accessible DNA ends, in vitro transcription, and an engineered Tn5 super-mutant (Sos et al., 2016)
Advantages:
- Requires very little input material
- Can detect small regions near distal enhancers
Disadvantages:
- Requires an engineered transposase
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
None available yet
References:
Sos B. C., Fung H. L., Gao D. R., et al. Characterization of chromatin accessibility with a transposome hypersensitive sites sequencing (THS-seq) assay. Genome Biol. 2016;17:20