PAT-ChIP

Pathology Tissue Chromatin Immunoprecipitation

PAT-ChIP is a variation of ChIP-Seq, optimized for the analysis of chromatin derived from FFPE samples. The distinguishing features of this method are dewaxing with a xylene substitute (Histolemon, Carlo Erba) followed by both MNase digestion and sonication (Fanelli et al., 2011). FiT-seq introduces further refinements to this method (Cejas et al., 2016)

Advantages:

  • Works on FFPE samples

Disadvantages:


Reagents:

Illumina Library prep and Array Kit Selector



Reviews:

Mimura I., Kanki Y., Kodama T. and Nangaku M. Revolution of nephrology research by deep sequencing: ChIP-seq and RNA-seq. Kidney Int. 2014;85:31-38



References:

Cejas P., Li L., O’Neill N. K., Duarte M., Rao P., et al. Chromatin immunoprecipitation from fixed clinical tissues reveals tumor-specific enhancer profiles. Nat Med. 2016;22:685-691