FiT-Seq
Fixed-Tissue Chromatin Immunoprecipitation Sequencing
FiT-seq is a method to extract soluble chromatin from FFPE tissue samples for the detection of histone-binding sites (Cejas et al., 2016). The distinguishing feature of this method is a proteinase K digestion step to reverse the effects of heavily crosslinked fixed chromatin in FFPE tissues, before the sonication step.
Advantages:
- Works on FFPE samples
- Higher resolution and sensitivity than pathology tissue chromatin immunoprecipitation (PAT-ChIP)
Disadvantages:
- Not tested in other laboratories
- Sonication may introduce sequence bias (Teytelman et al., 2009)
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
None available yet
References:
Cejas P., Li L., O’Neill N. K., et al. Chromatin immunoprecipitation from fixed clinical tissues reveals tumor-specific enhancer profiles. Nat Med. 2016;22:685-691