DNase I SIM
DNase I Simplified in-Nucleus Method for plants
This method is a simplified DNase I protocol specifically intended for plants(Cumbie et al., 2015). It contains an additional step of nuclei purification in Percoll gradients prior to DNase I digestion, in order to remove cellular debris and starch granules more efficiently. A DNA end-polishing step, using T4 DNA polymerase, is performed directly in the nuclei following DNase I digestion. These additional steps obviate the need for gel purification and its attendant loss of material.
Advantages:
- Does not require gel purification
Disadvantages:
- Optimized for plants only
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
None available yet
References:
Cumbie J. S., Filichkin S. A. and Megraw M. Improved DNase-seq protocol facilitates high resolution mapping of DNase I hypersensitive sites in roots in Arabidopsis thaliana. Plant Methods. 2015;11:42