ChIPmentation

Chromatin Immunoprecipitation with Sequencing Library Preparation by Tn5 Transposase

ChIPmentation combines ChIP with sequencing library preparation by Tn5 transposase (tagmentation) (Schmidl et al., 2015). Both ATAC-seq and ChIPmentation can be combined usefully in the same experiment to assay open chromatin and protein binding, respectively (Chaitankar et al., 2016). This combination of methods takes advantage of the flexibility and efficiency provided by using Tn5 transposase in library preparation.Tagmentation is performed directly on bead-bound, immunoprecipitated chromatin, followed by standard library preparation methods.

Advantages:

• Can generated accurate profiles from as little as 10,000 cells
• Simple, one-step reaction

Disadvantages:

• None reported

Reagents:

Illumina Library prep and Array Kit Selector



Reviews:

None available yet

References:

Rendeiro A. F., Schmidl C., Strefford J. C., Walewska R., Davis Z., et al. Chromatin accessibility maps of chronic lymphocytic leukaemia identify subtype-specific epigenome signatures and transcription regulatory networks. Nat Commun. 2016;7:11938

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History: ChIPmentation

Revision by sbrumpton on 2017-06-21 09:29:44 - Show/Hide