Covalent Attachment of Tags to Capture Histones and Identify Turnover

CATCH-IT is a direct method for measuring nucleosome turnover dynamics genome-wide (Deal et al., 2010). In this method, cells are treated briefly with the methionine surrogate azidohomoalanine (Aha), which couples biotin to nucleosomes containing newly incorporated histones. The labeled chromatin is affinity-purified with streptavidin, washed stringently to remove nonhistone proteins, and analyzed using tiling microarrays.


  • Can determine differences in nucleosome turnover across the genome


  • Potential artifacts


Illumina Library prep and Array Kit Selector


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