DSB-Seq
Map DNA Double-Strand Breaks
DSB-Seq maps in vivo DNA DSBs on a genome-wide scale (Baranello et al., 2014). In this method, DNA is biotinylated by a 3ê-end tailing reaction with biotin-16-dUTP. The biotinylated DNA is sonicated and captured with streptavidin-coated beads. The free DNA fragments are purified and used to prepare a sequencing library.
Advantages:
- Simple protocol
Disadvantages:
- Not yet adopted widely by the scientific community
Reagents:
Illumina Library prep and Array Kit Selector
Reviews:
None available yet
References:
Baranello L., Kouzine F., Wojtowicz D., Cui K., Przytycka T. M., et al. DNA break mapping reveals topoisomerase II activity genome-wide. Int J Mol Sci. 2014;15:13111-13122