Map DNA Double-Strand Breaks
DSB-Seq maps in vivo DNA DSBs on a genome-wide scale (Baranello et al., 2014). In this method, DNA is biotinylated by a 3ê-end tailing reaction with biotin-16-dUTP. The biotinylated DNA is sonicated and captured with streptavidin-coated beads. The free DNA fragments are purified and used to prepare a sequencing library.
- Simple protocol
- Not yet adopted widely by the scientific community
Illumina Library prep and Array Kit Selector
None available yet
Baranello L., Kouzine F., Wojtowicz D., Cui K., Przytycka T. M., et al. DNA break mapping reveals topoisomerase II activity genome-wide. Int J Mol Sci. 2014;15:13111-13122